Figure 4: Detection of POSTN and β-catenin expression in the tissue engineering xenograft.

(A) Histological analysis by H&E and Masson’s Trichrome staining (circle: DBM; pentagram: new bone formation area) of newly formed bone, and the expressions of POSTN and β-catenin detected by immunohistochemistry at 2 months post implantation (arrow: positive expression). Scale bars: 500 μm. (B,C) The IOD of POSTN and β-catenin was quantified by Image-Pro Plus 6.0. n = 3, *P < 0.05.