Figure 5: POSTN expression in an immune activation condition in vitro.

(A) The localization of POSTN expression in MSCs and CTLA4-modified MSCs was detected by immunohistochemistry. Scale bars: 100 μm. (B) MSCs or CTLA4-modified MSCs were cultured in the absence (−) or presence (+) of PHA or PBMCs for 3 days as indicated, and POSTN expression was detected by western blotting (20 μg each). (C) POSTN mRNA levels were quantified by real-time PCR. n = 3, *P < 0.05.