Table 1 L-asparaginase kinetic parameters for WoA-S121 and WoA-P121.

From: The differential ability of asparagine and glutamine in promoting the closed/active enzyme conformation rationalizes the Wolinella succinogenes L-asparaginase substrate specificity

Enzyme

kcat (sec−1)

Km (μM)

kcat/Km (sec−1μM−1)

kobs@50 μM a (sec−1)

WoA-S121

97.8 ± 1.0

21.7 ± 1.2

4.5 ± 0.25

67.8 ± 0.4

WoA-P121

86.7 ± 0.8

23.6 ± 1.1

3.7 ± 0.17

59.5 ± 0.8

  1. akobs@50 μM is the turnover rates at the physiologically concentration of Asn.