Figure 2: rhCygb identification profile and biological activity assessment. | Scientific Reports

Figure 2: rhCygb identification profile and biological activity assessment.

From: Protective effects of recombinant human cytoglobin against chronic alcohol-induced liver disease in vivo and in vitro

Figure 2

(a) SDS-PAGE analysis of rhCygb expression: Lane 1, Protein marker; Lane 2, Induced E. coli cell lysate; Lane 3, Uninduced E. coli cell lysate clone. (b) Purification of rhCygb: Lane 1, Protein marker; Lane 2, Purified protein by sephadex G-25; Lane 3, Purified protein by affinity chromatography. (c) Western blot analysis of purified rhCygb: Lane 1, Protein marker; Lane 2, Purified rhCygb; Lane 3, Cygb knockout cell lysate. (d) Biological activity assessment of rhCygb: The activity of rhCygb was significantly (*P < 0.01) higher than the controls (SIRT2 and PBS) at the same concentrations. (The gel of Fig. 2a,b has been cropped, the full-length gels are presented in Supplementary figure 2a and Fig. 2b. The blot of Fig. 2c has not been cropped, the original blot is presented in Supplementary Figure 2c).

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