Figure 2: Effect of NLS on the viability of prostate cancer cells.

(A) Prostate cancer cell lines PC3, DU145, C4-2B, RM1, and immortalized human normal prostatic epithelial cell line RWPE-1 were treated with NLS at concentrations of 0 to 250 μg/ml in triplicates for 24, 48 and 72 h. Cell viability was measured by MTS assay. (B) Evaluation of the clonogenic growth potential of PC3 and DU145 cells treated with NLS for 8 days. At the end of incubation, colony formation was observed by staining with crystal violet. (C) Bar plot of the average colony number of PC3 and DU145 cells. Similar results were obtained in 3 independent experiments. Data are expressed as mean ± SD. Compared with control group: **p < 0.01.