Figure 2: Immunoassay on rice CenH3 binding-specificity and FISH analyses of CenH3 ChIP DNA enrichment and localizations.
From: Isolation and characterization of centromeric repetitive DNA sequences in Saccharum spontaneum

(A) Metaphase chromosome spread from a S. spontaneum SES208 root tip cell. (B) Immunofluorescence signals detected at the primary chromosome constriction. (C) Merged image of (A,B). (D) Metaphase chromosome spread from a S. spontaneum SES208 root tip cell. (E) FISH signals of CenH3 ChIP DNA probe (F) Merged image of (D,E). (G) Metaphase chromosome spread from a S. spontaneum SES208 root tip cell. (H) FISH signals of mock DNA probe.(I) Merged image of (G,H). Bar = 10 μm.