Figure 1: Platelet counts and platelet activation during different days of fever in dengue patients.
From: Platelet activation determines the severity of thrombocytopenia in dengue infection
(A) Platelet number was measured from freshly isolated blood samples of dengue patients (n = 19) at different days of fever (4, 6, 8 and 10) and of healthy controls (n = 10). Dot represents median value for each individual from three separate readings. Low platelet count was observed at day (d) 4 and rescued at d10, ***p < 0.0007 d4 vs d10, ***p < 0.0003 d6 vs d10, ns = non-significant d8 vs d10 (Mann-Whitney test). (B) P-selectin expression, (C) PAC1 binding (D) PS exposure on platelets were measured from freshly isolated platelets from patients and normal individuals by flow cytometry. All three parameters show elevated level at d4 and decreased further at d10 (P-selectin: ***p < 0.0003 d4 vs d10, ***p < 0.0004 d6 vs d10 and **p < 0.0027d8 vs d10; PAC1: ***p < 0.0005 d4 vs d10, ***p < 0.0002 d6 vs d10 and **p < 0.0018 d8 vs d10; PS: ***p < 0.0001, d4/6/8 vs d10). (E) Platelet-derived microparticles (MPs) in plasma were measured by flow cytometry using CD41a antibody. Plasma MPs level was highest at d4 and lowest at d10 (***p < 0.0004 dv vs d10, ***p < 0.0002 d6 vs d10 and ***p < 0.0008 d8 vs d10). Data of healthy individuals is mentioned as normal reference. The detail of each patient’s data is mentioned in suppl. Table-1.
