Figure 6: Platelet thrombus and platelet-VWF string formation on HUVEC monolayer under flow shear in vitro in presence of DENV2.
From: Platelet activation determines the severity of thrombocytopenia in dengue infection
Whole blood of healthy individual was incubated with or without DENV2 (3.4 MOI) and perfused over HUVEC monolayer under shear of 2.5 dyne/cm2 in presence of mepacrine (label platelets). (A) Platelet thrombus area and (B) platelet-VWF string number and (C) string length were measured. Data are the mean ± SEM from ten view-fields in three independent experiments. The thrombus area, and string number and length were increased in presence of DENV2, *p < 0.05. The string length (calculated as median with highest and lowest value) was increased in presence of DENV2, **p < 0.005. (D) The 40X images show the platelet thrombus (marked by dotted area) and platelet- VWF string (marked by arrow) in absence (D1) and presence (D2) DENV2.
