Figure 1: Coronin 1A was decreased during osteoclast differentiation. | Scientific Reports

Figure 1: Coronin 1A was decreased during osteoclast differentiation.

From: Actin-binding protein coronin 1A controls osteoclastic bone resorption by regulating lysosomal secretion of cathepsin K

Figure 1

(a,b) The expression of coronin 1A during osteoclastogenesis. The bone marrow macrophages (BMMs) were cultured in the presence of macrophage colony-stimulating factor (M-CSF) (10 ng/mL) and receptor activator of nuclear factor kappa-B ligand (RANKL) (50 ng/mL) for the indicated times, and analysed Coro1a mRNA (a) and coronin 1A protein level (b). Unprocessed original scans of blots are shown in Supplementary Fig. 10. *P < 0.05, **P < 0.01, (Student’s t-test). Data on Coro1a mRNA expression are means ± standard deviation (SD) of three independent experiments. (c) The intracellular localization of coronin 1A in osteoclasts. The osteoclasts were fixed, stained with anti-coronin 1A antibody, DAPI and phalloidin, and observed by confocal microscopy. Scale bars, 20 μm. Data are representative of three experiments.

Back to article page