Figure 3: RBM3 overexpression prevents SH-SY5Y cells from NO-induced apoptosis. | Scientific Reports

Figure 3: RBM3 overexpression prevents SH-SY5Y cells from NO-induced apoptosis.

From: RNA-binding protein RBM3 prevents NO-induced apoptosis in human neuroblastoma cells by modulating p38 signaling and miR-143

Figure 3

(A) SH-SY5Y cells were transfected with plasmid pXJ40-myc (Veh) or pXJ40-myc-RBM3 (RBM3) for 2 d, and Western blotting was performed to confirm the overexpression of RBM3. β-actin served as a loading control. (B–E) 2 d post plasmid transfection, cells were insulted with 1 mM SNP for 16 h. (B) Cell viability was assessed by MTT assay, and (C) cleaved PARP were detected by Western blotting. (D) Histogram analysis for the relative levels of cleaved PARP in panel C. Results were normalized by β-actin. (E) Fluorescence-activated cell sorting (FACS) analysis was conducted to evaluate the effect of RBM3 overexpression on NO-induced apoptosis. ***P < 0.001 versus control group.

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