Figure 1: Detergent quantification by MALDI-TOF MS. | Scientific Reports

Figure 1: Detergent quantification by MALDI-TOF MS.

From: Quantification of Detergents Complexed with Membrane Proteins

Figure 1

(a) Principle of the method. Components are colored in blue, orange and green for the measured detergents, standard detergents and the desorption-helper matrix, respectively. (b) Detergent desorption. Detergent m/z are indicated, plus the mass of Na when NaI was added to the mixture. The different combinations of assayed and standard detergents are displayed from top to bottom, with respect to the ratios indicated on the left of the panel. (c) Abundance distribution from 3 independent experiments displayed in light to dark colors, blue-type for measured detergents and orange-type for standards. (d) Calibration curves, plotted as the amount of measured detergent (%, w/v) with respect to assayed/standard detergent abundance ratios. Circle, square and diamond correspond to 3 experiments, fitted with a linear regression.

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