Figure 3: Compared quantification of DDM bound to the SERCA1a Ca2+-ATPase.
From: Quantification of Detergents Complexed with Membrane Proteins

The protein was extracted with radiolabeled (upper panel) or cold (lower panel) DDM and then submitted to two successive SEC. Green traces correspond to the absorbance at 280 nm. 14C-DDM was quantified by liquid scintillation (pink stars) and 12C-DDM by MALDI-TOF MS (blue bars). Quantifications were done in triplicate on the same experiment.