Figure 1: Downregulation of MCM7 induces γH2AX over-expression in tamoxifen-resistant breast cancer cells under the condition of an Rb defect. | Scientific Reports

Figure 1: Downregulation of MCM7 induces γH2AX over-expression in tamoxifen-resistant breast cancer cells under the condition of an Rb defect.

From: Simvastatin suppresses the DNA replication licensing factor MCM7 and inhibits the growth of tamoxifen-resistant breast cancer cells

Figure 1

(A,B) Both MCF7 and T47D cells were infected with HPV E7 adenovirus (Ad E7) or control adenovirus (Ad CON) for 24 h and were then transfected with MCM7 siRNA or non-target siRNA (siNT). Forty-eight hours after transfection, the cells were lysed, and western blot was performed. (C) The MCF7 and T47D cells were infected with HPV E7 adenovirus (Ad E7) or control adenovirus vector (Ad CON) for 24 h and were then transfected with MCM7 siRNA. Forty-eight hours after transfection, the cells were fixed, and γH2AX was stained by immunofluorescence. The bars represent 50 μm. (D) The expression of p-RB, RB and MCF7 in wild-type cells and tamoxifen-resistant cells. (E) MCF7 TamR and T47D TamR cells were transfected with MCM7 siRNA or non-target siRNA (siNT). Forty-eight hours after transfection, the cells were lysed, and a western blot was performed.

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