Figure 3: LIVE/DEAD fluorescence images of S. mutans biofilms on material surfaces with or without aging. | Scientific Reports

Figure 3: LIVE/DEAD fluorescence images of S. mutans biofilms on material surfaces with or without aging.

From: Antibacterial Activity and Bonding Ability of an Orthodontic Adhesive Containing the Antibacterial Monomer 2-Methacryloxylethyl Hexadecyl Methyl Ammonium Bromide

Figure 3

Representative CLSM images of LIVE/DEAD stained S. mutans biofilms after 24 h of anaerobic growth on the tested material surfaces: (A) Transbond XT, (B) Transbond XT+1%MAE-HB, and, (C) Transbond XT+3%MAE-HB, (D) Transbond XT+5%MAE-HB. Streptococcus mutans biofilms on the corresponding aged samples are shown in E–H. Live bacteria were stained green, and the compromised bacteria were stained red. When the live and dead bacteria are close to each other or on the top of each other, the yellowish or orange fluorescence is presented. Scale bars, 500 μm.

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