Figure 2: MYH3 is expressed embryonically and postnatally in muscles joining the neural arches and postnatally in bone. | Scientific Reports

Figure 2: MYH3 is expressed embryonically and postnatally in muscles joining the neural arches and postnatally in bone.

From: A postnatal role for embryonic myosin revealed by MYH3 mutations that alter TGFβ signaling and cause autosomal dominant spondylocarpotarsal synostosis

Figure 2

(A) Sagittal paraffin sections of spines at E15.5, P1, and P15 stained via IHC using an antibody against MYH3. N = 3 biological replicates. First column: H&E staining for morphology. Second column: IHC staining for MYH3. Third column: Negative control for IHC stain. IVD: MYH3 is not expressed in the developing IVD at E15.5, P1, or P15. MYH3 expression can be seen postnatally in the bone. Cervical and Thoracic Neural Arches: At E15.5, P1 and P15, MYH3 is expressed in the muscle between the neural arches of the cervical and thoracic spine. At P15, MYH3 expression is diminished but continues to be highly localized between the neural arches. IVD = Intervertebral Disc, NP = Nucleus Pulposus, AF = Annulus Fibrosus, NA = Neural Arch. (B) RT-qPCR using primers against MYH3 in skeletal muscle, the annulus fibrosus and the nucleus pulposus at P15. MYH3 is expressed in skeletal muscle but not expressed in the annulus fibrosus or the nucleus pulposus.

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