Figure 3: TGFβ and BMP signaling pathways are altered in cells transfected with mutated MYH3. | Scientific Reports

Figure 3: TGFβ and BMP signaling pathways are altered in cells transfected with mutated MYH3.

From: A postnatal role for embryonic myosin revealed by MYH3 mutations that alter TGFβ signaling and cause autosomal dominant spondylocarpotarsal synostosis

Figure 3

(A) Western blot of HEK cells transfected with WT and mutant forms of the MYH3 plasmid indicating that all mutated plasmids expressed a protein. The plasmid harboring the p.Leu900fs9 mutation produced a truncated protein at ~100 kDa (arrow). (B) Western blot of HEK cells transfected with WT and mutant forms of the MYH3 plasmid stimulated with TGFβ-1 ligands. (C) Quantitation of protein stability (n = 6). (D–F) Quantitation of Western blot results normalized to GAPDH levels as well as transfected MYH3 protein levels. Because Western blots for each biological replicate were performed separately, samples transfected with the mutated plasmids or no plasmid were analyzed as ratios against samples transfected with the WT plasmid, with the WT plasmid samples set at a value of 1. Data were analyzed by Student’s T-test; the results are shown as the mean ± standard error of n = 3 biological replicates. P ≤ 0.05 was considered statistically significant.

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