Figure 5: Analysis of the BD-HLCs treated with 4-phenylbutyrate. | Scientific Reports

Figure 5: Analysis of the BD-HLCs treated with 4-phenylbutyrate.

From: Generation of a bile salt export pump deficiency model using patient-specific induced pluripotent stem cell-derived hepatocyte-like cells

Figure 5

The BD1, BD2, and Control HLCs were treated with 1 mM 4PBA for 24 hours. (A,B) Gene expression levels of ALB and BSEP in the HLCs as examined by real-time RT-PCR. The gene expression levels in solvent-treated HLCs were taken as 1.0. Data represent mean ± SD (n = 3). (C) Immunofluorescence staining of BSEP in the HLCs. The scale bar represents 20 μm. (D) BEI of 4PBA-treated HLCs. Data represent mean ± SE (three independent differentiation experiments). Statistical significance was evaluated by Student’s t-test (n = 3). *P < 0.05.

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