Figure 1: CsAYTE promotes commitment of PSCs into CLCs.

(A) Protocol for the commitment of Flk1⁺ MPCs into CLCs induced by four specific modulators in an OP9 co-culture system. LIF, Leukemia inhibitory factor. (B and C) Representative FACS analysis and the percentage of mouse ESC-derived cTnT⁺ cells incubated with the indicated agents. Con, control vehicle; Y, Y27632 (10 μM); T, Trolox (400 μM); E, EW7197 (1 μg/mL); CsA (3 μg/mL). Each group, n = 4. (D and E) Images displaying α-actinin⁺ cells, DAPI⁺ nuclei and αMHC-GFP⁺ cells (Scale bars, 100 μm), Inset: High resolution confocal image indicating sarcomeric structure (Scale bars, 5 μm), and comparison of α-actinin⁺ area (%) and αMHC-GFP⁺ area (%). Each group, n = 3–4. (F and G) Representative FACS analysis and percentage of mouse iPSC-derived cTnT⁺ cells grown in OP9 co-culture. Each group, n = 4. In C, E, G graphs, *p < 0.05 and **p < 0.01 versus Con; ^#p < 0.05 and ^##p < 0.01 versus CsA. (H and I) Representative FACS analysis and percentage of mouse ESC-derived cTnT⁺ cells grown in a feeder-free culture with or without Wnt signaling inhibitor IWR-1 (2 μM). Each group, n = 5. **p < 0.01 versus Control.