Figure 4: Human PSCs differentiates into PCBs under CsAYTE stimulation.
From: Generation of PDGFRα+ Cardioblasts from Pluripotent Stem Cells
(A) Protocol to generate PCBs in human iPSCs by CsAYTE stimulation. (B) Phase-contrast images showing differentiating MPCs at day 4.0 from human iPSCs incubated with Control, CsA, and CsAYTE. Scale bars, 100 μm. (C and D) Representative FACS analysis and quantification of PDGFRα+ VEGFR2− cells at day 6.0 from human iPSCs incubated with Control, CsA, and CsAYTE. Each group, n = 6. (E and F) Representative FACS analysis and percentage of human iPSC-derived cTnT+ cells grown in feeder-free culture at day 10.5. Each group, n = 3–4. (G and H) Images displaying human iPSC-derived cTnT+ cells at day 10.5 and the quantification analysis of cTnT+ area (%). Each group, n = 3. In all graphs, *p < 0.05 and **p < 0.01 versus Con; #p < 0.05 versus CsA. Scale bars, 100 μm. (I) Percentage of cTnT+ cells at day 10.5 after sorting of PCBs at day 4.0. Each group, n = 4. *p < 0.05 versus PCB pool.
