Figure 1: Spontaneous generation of autoantibodies related to connective tissue autoimmune diseases in P-Sel−/− mice.
From: P-Selectin preserves immune tolerance in mice and is reduced in human cutaneous lupus
(a) Representative immunofluorescence photomicrographs of HEp-2 cells incubated with serum from 2 independent wild-type (WT) and 2 independent P-Sel−/− mice. (b) Percentage of mice positive for anti-Sm, RNP, Scl-70 and Jo-1 autoantibodies (n = 8–10 animals per group); *p < 0.05 by Chi-square test. (c) Percentage of mice positive for anti-dsDNA autoantibodies (n = 8–10 animals per group); *p < 0.05 by Chi-square test. Immunofluorescence photomicrographs of C. luciliae incubated with serum of a P-Sel−/− mouse (right panel). (d) Photograph of representative spleens of 3-month-old WT and P-Sel−/− mice (upper panel). Spleen weight/body weight ratio of female and male 3-month-old WT and P-Sel−/− mice (middle panel) (n = 6 mice per group). Total number of cells per spleen of WT and P-Sel−/− mice (lower panel). (e,f) Percentage of splenic follicular T helper (Tfh) cells (e) and germinal center (GC) B cells (f) in 3-month-old male WT and P-Sel−/− mice. *p < 0.05; ***p < 0.005 by Student’s two-tailed t-test. Bars show the mean ± standard deviation (SD).
