Figure 2: Peripheral blood and spleen immune system characterization in P-Sel−/− mice.
From: P-Selectin preserves immune tolerance in mice and is reduced in human cutaneous lupus
(a) Relative frequency of peripheral blood leukocyte populations of 1.5–3 month-old WT and P-Sel−/− mice. (b,d) Percentage of IL-10+ conventional dendritic cells (cDC), plasmacytoid DC (pDC), monocytes, granulocytes and B cells; and frequency of IL-10 and IL-17 producing CD4+ and CD8+ T lymphocytes, in 1.5-month-old (b) and >18-month-old (d) WT and P-Sel−/− mice. (c) Representative dot plots of IL-10+ cDCs and IL-10 and IL-17 producing CD4+ T cells in 1.5-months old WT and P-Sel−/− mice. (e) Phenotyping of CD4+ and CD8+ splenic T lymphocytes according to the expression of the naïve/memory/effector markers CD62L and CD44 in 1.5–3 months-old (upper panels) and >18 month-old (lower panels) WT and P-Sel−/− mice. (f) Representative dot plots showing the distribution of 1.5–3-month-old mice splenic populations according to the expression of L-Selectin and CD44. In all cases, n = 4 mice per group. In all cases, n = 4 mice per group. Bars represent the mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.005, by Student’s two tailed t test.
