Figure 4: Histological alterations in the skin of P-Sel−/− mice.
From: P-Selectin preserves immune tolerance in mice and is reduced in human cutaneous lupus
(a) Photomicrographs (10×) of hematoxylin and eosin (H&E)-stained skin sections of 1.5 month-old (upper panels) and 24-month-old female and male WT and P-Sel−/− mice (lower panels). Blue arrowheads show panniculitis. Black arrowheads show acanthosis; arrows show hyperkeratosis and keratin plugs. Scale bars represent 200 μm. (b) Quantification of dermis, epidermis and corneal layer width of WT and P-Sel−/− mice. (c) Pathological activity index of skin samples obtained from WT and P-Sel−/− mice. (d) Lesions developed in the back of UV-irradiated 3-month-old female WT and P-Sel−/− mice. (e) Photomicrographs (5×) of representative skin sections of UV-irradiated 3-month-old female WT and P-Sel−/− mice. n = 4 mice per genotype. Representative experiment of three independent replicates. Scale bars represent 500 μm. (f) Pathological activity index of skin samples obtained from UV-irradiated 3-month-old female WT and P-Sel−/− mice. (b,c,f) Bars show the mean ± SD *p < 0.05; **p < 0.01; ***p < 0.005 by Student’s two tailed t test. n = 8–10 mice per genotype.
