Figure 5: Expression analysis and subcellular localization of Fd-GOGAT protein.

(A) The expression of Fd-GOGAT in different tissues. Leaf was selected from seedling of 30 days after sowing of the wild-type 9311; root, flag leaf, panicle, and leaf sheath were selected from the wild-type 9311 at heading stage. (B) The expression of Fd-GOGAT at three different developmental stages. The fourth leaves of 15, 23, 31 days after sowing in wild-type 9311 and spl32 mutant were selected. Data are means ± SD (n = 5). Asterisks indicate the statistical significance levels according to Student’s t test: **P < 0.01 and *P < 0.05. (C) GFP signals of the Fd-GOGAT-GFP fusion protein located in the chloroplasts of rice protoplasts. (D) GFP signals were dispersed in rice protoplasts. Bars = 10 μm.