Figure 7: Dying experiments and the determination of enzyme activities involved in scavenging and generating mechanism of ROS. | Scientific Reports

Figure 7: Dying experiments and the determination of enzyme activities involved in scavenging and generating mechanism of ROS.

From: Isolation and characterization of a spotted leaf 32 mutant with early leaf senescence and enhanced defense response in rice

Figure 7

(A) non-spotted leaves and serious spotted leaves of spl32 mutant and the corresponding sections of leaves in the wild-type 9311 at tillering stage were stained by trypan blue and 3, 3-diaminobenzidine (DAB) respectively. Controls were not stained and decolorized directly by alcohol. (B) The contents of hydrogen peroxide (H2O2) and malonaldehyde (MDA) in the wild-type 9311 and spl32 mutant at tillering stage. FW, fresh weight. (C) The enzyme activities of catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (POD), and peroxidase (APX) in wild-type 9311 and spl32 mutant at tillering stage. FW, fresh weight. (D) The enzyme activities of NADPH oxidase and polyamine oxidase (PAO) in wild-type 9311 and spl32 mutant at tillering stage. FW, fresh weight. Data represent means ± SD of three independent measurements. Asterisks indicate the statistical significance levels according to Student’s t test: **P < 0.01 and *P < 0.05. FW, fresh weight.

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