Figure 3: Characterization of iPSCs in vitro. | Scientific Reports

Figure 3: Characterization of iPSCs in vitro.

From: EpEX/EpCAM and Oct4 or Klf4 alone are sufficient to generate induced pluripotent stem cells through STAT3 and HIF2α

Figure 3

(A) Flow cytometry was performed to detect Oct4, Sox2, Nanog, and SSEA1 (n = 3). (B) Immunofluorescent staining was performed for the detection of Oct4, Sox2, Nanog, and SSEA1 by confocal microscopy (n = 3). Scale bar: 50 μm. (C) Western blotting for the detection of Oct4, Sox2, and Nanog (n = 3). (D) Q-PCR analysis (n = 3). Q-PCR analysis was used to show that the iPSCs express endogenous pluripotency genes, including Oct4, Sox2, Klf4, c-Myc, Nanog, Spalt-Like Transcription Factor 4 (Sall4), dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1 (DAXR1), developmental pluripotency-associated 5 (Dppa5), zinc finger protein (ZFP), undifferentiated embryonic cell transcription factor (UTF), cysteine-rich PDZ-binding protein (CRIPTO), reduced expression (REX). The parental MEF group was used as a control. (E) me3H3K27 immunostaining of MEFs and iPSCs. Red color indicates the staining of me3H3k27. Blue color indicates the DAPI staining. Scale bar: 10 μm.

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