Figure 4: Characterization of iPSCs in vivo. | Scientific Reports

Figure 4: Characterization of iPSCs in vivo.

From: EpEX/EpCAM and Oct4 or Klf4 alone are sufficient to generate induced pluripotent stem cells through STAT3 and HIF2α

Figure 4

(A) Teratoma formation (n = 3). Three germ layers (ectoderm, endoderm, and mesoderm) were detected in all iPSCs. Scale bar: 50 μm. (B) Genotyping of chimeras. Single iPSC clones were picked and amplified for each group. We injected 6–11 iPSCs into a 3.5 day-old blastocyst (n = 3). The GFP signals represent chimerism in multiple tissues. (C) The urogenital ridge in 13.5 day embryos was observed under fluorescence microscopy. Oct4 signals from iPSCs were detected in the urogenital ridge (n = 3).

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