Figure 6: EpEX and EpCAM activate a STAT3-HIF2α signal for EpEX/EpCAM-mediated iPSC formation. | Scientific Reports

Figure 6: EpEX and EpCAM activate a STAT3-HIF2α signal for EpEX/EpCAM-mediated iPSC formation.

From: EpEX/EpCAM and Oct4 or Klf4 alone are sufficient to generate induced pluripotent stem cells through STAT3 and HIF2α

Figure 6

(A) iPSCs were infected with two different EpCAM shRNAs (two clones, #1 and #2). The protein expressions of EpCAM and HIF2α were detected by Western blotting. (B) MEFs were stimulated by EpEX (1 μg/mL) at the indicated times. Nuclear-translocation was detected with a specific antibody against HIF2α (n = 3). (C) Immunofluorescence staining was performed to detect subcellular localization of HIF2α. Nuclei were stained with DAPI. Scale bar: 10 μm. (D) MEFs were treated with STAT3 inhibitor (WP1066, 10 μM), and then stimulated with EpEX for 30 min. The nuclear-translocation of HIF2α was detected by Western blotting with anti-HIF2α antibody (n = 3). (E) iPSC morphology was observed at day 20 after induction. Reprogramming of Oct4-GFP MEFs was induced by transfection of OSKM, OE + EpEX, and KE + EpEX with or without STAT3 inhibitor WP1066, or HIF2α shRNA (n = 3). Scale bar: 50 μm.

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