Table 1 PCR Primers Used in the Gene Expression Analysis.

From: Toxic metabolites, MAPK and Nrf2/Keap1 signaling pathways involved in oxidative toxicity in mice liver after chronic exposure to Mequindox

Gene name

Description

Sequence (5′-3′)

Length (bps)

β-actin

mβ-actin - F

CTGTCCCTGTATGCCTCTG

221

mβ-actin - R

TTGATGTCACGCACGATT

P38

mP38 - F

GGAGAAGATGCTCGTTTTGGA

211

mP38 - R

TTGGTCAAGGGGTGGTGG

JNK

mJNK - F

TCTCCAGCACCCATACATCAA

150

mJNK - R

TCCTCCAAATCCATTACCTCC

Nrf-2

mNrf-2 - F

TCCTATGCGTGAATCCCAAT

103

mNrf-2 - R

GCGGCTTGAATGTTTGTCTT

Keap1

mKeap1 - F

GATCGGCTGCACTGAACTG

106

mKeap1 - R

GGACTCGCAGCGTACGTT

NF-κB

mNF-κB - F

GGTGGAGGCATGTTCGGTA

142

mNF-κB - R

TGACCCCTGCGTTGGATT

NQO1

mNQO1 - F

TTCTGTGGCTTCCAGGTCTT

104

mNQO1 - R

TCCAGACGTTTCTTCCATCC

GCLC

mGCLC - F

ATGTGGACACCCGATGCAGTATT

200

mGCLC - R

GTCTTGCTTGTAGTCAGGATGGTTT

HO-1

mHO-1 - F

GACAGAAGAGGCTAAGACCGC

213

mHO-1 - R

TGGAGGAGCGGTGTCTGG

  1. Note: The primers were manufactured by Nanjing Genescript Co. Ltd. (Nanjing, PR China). P38, p38 MAP Kinase; JNK, c-Jun N-terminal protein kinase; Nrf-2, NF-E2-related factor 2; Keap1, Kelch-like ECH-associated protein1; NF-κB, nuclear factor κB; NQO1, NAD(P) H: quinoneoxidoreductase; HO-1, heme oxygenase 1; GCLC, catalytic subunit of glutamate-cysteine ligase.
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