Figure 3: IMB-6G blocks autophagic flux in the degradation stage. | Scientific Reports

Figure 3: IMB-6G blocks autophagic flux in the degradation stage.

From: Lysosomal dysfunction and autophagy blockade contribute to IMB-6G-induced apoptosis in pancreatic cancer cells

Figure 3

(a) MiaPaCa-2 and HupT-3 cells were pretreated with CQ (50 μM) or rapamycin (200 nM) for 2 hours, followed by IMB-6G (5 μM) treatment for 12 h. The LC3 turnovers in both cells were detected by immunoblotting. (b) MiaPaCa-2 cells were transfected with mCherry-EGFP-LC3 plasmid, followed by treatment with IMB-6G (5 μM) or CQ (50 μM) for 6 h. Representative fluorescent images are visualized with confocal microscopy (scale bars, 20 μm). (c) Quantification of GFP/mCherry double-positive and mCherry single-positive puncta per cell in control or cells treated with CQ or IMB-6G. Data were the mean value of three independent experiments with each count of no less than 100 cells. Values are expressed as the mean ± SD, *p < 0.05, **p < 0.01 vs. untreated control.

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