Figure 5: IMB-6G induced autophagy-dependent LMP. | Scientific Reports

Figure 5: IMB-6G induced autophagy-dependent LMP.

From: Lysosomal dysfunction and autophagy blockade contribute to IMB-6G-induced apoptosis in pancreatic cancer cells

Figure 5

(a) MiaPaCa-2 cells were treated with 2 μM IMB-6G for 6 h, 12 h, or 24 h. Lysosomal membrane stability was measured by AO staining under a fluorescence microscopy. CQ (50 μM) was used as a positive control (scale bars, 20 μm). (b) Quantification of red and yellow fluorescence intensity of AO in MiaPaCa-2 cells. Data were the mean value of three independent experiments with each count of no less than 100 cells. Values are expressed as the mean ± SD, **p < 0.01 vs. untreated control. (c–e) MiaPaCa-2 cells were transfected with either 50 nM siRNA against human Atg5 (siAtg5) or control siRNA (siCtrl), and then treated with 5 μM IMB-6G for 12 h. (c) siRNA transfection efficiency was assessed by immunoblotting after 48 h of transfection. β-actin was used as an internal control. (d) Lysosomal membrane stability was measured by AO staining under a fluorescence microscopy (scale bars, 20 μm). (e) The quantification of red and yellow fluorescence intensity of AO was shown. Values are expressed as the mean ± SD, #p < 0.01 compared with IMB-6G-untreated siCtrl group, *p < 0.05, **p < 0.01 compared with IMB-6G-treated siCtrl group.

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