Figure 6: Inhibition of autophagosome formation alleviated IMB-6G-induced lysosomal release of cathepsin. | Scientific Reports

Figure 6: Inhibition of autophagosome formation alleviated IMB-6G-induced lysosomal release of cathepsin.

From: Lysosomal dysfunction and autophagy blockade contribute to IMB-6G-induced apoptosis in pancreatic cancer cells

Figure 6

(a) MiaPaCa-2 cells were treated with 5 μM IMB-6G for 12 h, cell fragments were performed to separate lysosomal and cytosolic fraction from DMSO- or IMB-6G-treated cells. CTSB and CTSD levels were detected by immunoblotting of the different fractions. Lysosomal-associated membrane protein 1(LAMP1) was as a lysosomal marker, β-actin was a cytosolic marker. (b) MiaPaCa-2 cells were transfected with control siRNA or siAtg5, followed by IMB-6G treatment for 12 h, lysosomal and cytosolic fraction were separated and CTSB, CTSD levels were determined by immunoblotting.

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