Figure 6
From: Mechanisms underlying the cardiac antifibrotic effects of losartan metabolites
Histograms depict the fold change in LOX mRNA, protein and activity in HDFa fibroblasts stimulated without and with 10−4 μg/mL TGF-β1 for 24 hours, in the absence or the presence of 1, 2, 5, 10, 20 and 50 μM of EXP3179 (panels A–C) and EXP3174 (panels D–F), or incubated with the two compounds alone at 20 and 50 μM, as compared to control cells. Line graphs show percentage of inhibition in LOX mRNA (panel G), protein (panel H), and activity (panel I), in HDFa stimulated with 10−4 μg/mL TGF-β1 in combination with EXP3174 (□) or EXP3179 (∆) at the above mentioned concentrations, compared to cells incubated with TGF-β1 alone. Bars represent mean +SEM (n = 5 to 8 experiments) and curves show means ± SEM (n = 5 to 8). *P < 0.05 vs Control, †P < 0.05 vs TGF-β1, ††P < 0.01 vs TGF-β1 and $P < 0.05 vs EXP3174.
