Figure 9: ADAMTS17-PCD interferes with fibrillin-2 microfibril formation by mouse embryo fibroblasts. | Scientific Reports

Figure 9: ADAMTS17-PCD interferes with fibrillin-2 microfibril formation by mouse embryo fibroblasts.

From: Unusual life cycle and impact on microfibril assembly of ADAMTS17, a secreted metalloprotease mutated in genetic eye disease

Figure 9

(a) Mouse embryo fibroblasts (MEFs) were cultured in the presence of 50 μg ADAMTS17-PCD (PCD) or an equivalent volume of buffer (PBS) for 48 h and co-stained for ADAMTS17-PCD with anti-myc and with antibodies against fibrillin-1 (FBN1), fibrillin-2 (FBN2), or fibronectin (FN) as indicated. (b) qRT-PCR analysis of Fbn1, Fbn2, and Fn in MEFs shows reduction in Fbn2 gene expression in the presence of ADAMTS17-PCD (PCD). Note the lesser reduction in Fbn1 expression and no change in Fn gene expression. (c) MEF cell number was greatly reduced in the presence of ADAMTS17-AD. (d) Rat aortic smooth muscle cells (ASMC, A7r5) were cultured in the presence of ADAMTS17-PCD (PCD), ADAMTS17-AD (AD) or the equivalent volume of PBS for 72 h and costained using anti-myc and fibronectin (FN). Note the punctate staining of ADAMTS17-PCD and ADAMTS17-AD, which does not co-localize with fibronectin fibrils.

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