Figure 3: Reporter gene expression is stable and restricted to hepatocytes after pBDL following neonatal intravascular administration of lentiviral vectors. | Scientific Reports

Figure 3: Reporter gene expression is stable and restricted to hepatocytes after pBDL following neonatal intravascular administration of lentiviral vectors.

From: Longitudinal in vivo bioimaging of hepatocyte transcription factor activity following cholestatic liver injury in mice

Figure 3

SFFV-FLuc/eGFP lentivirus was administered to P1 neonatal mice by intravascular (i.v.) injection and then subjected to pBDL or sham pBDL in adulthood. (A) Mice were subjected to continued luciferase bioimaging over 40 days where no change in luciferase activity was observed over time or between pBDL and sham groups (n = 10 pBDL, 5 sham, not significant, Student’s t-test). Mice were sacrificed 90 days after pBDL and co-immunostained for GFP and markers of (Bi-iii) hepatocytes; HNF4α, (C-iii) biliary epithelia; CK7, (Di-iii) hepatic progenitors; PKM2, (Ei-iii) myofibroblasts; αSMA and (Fi-iii) hepatic stellate cells; GFAP (all groups n = 3–6).

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