Figure 7: In vitro exposure of NCS to the human cell line MCF-7, the bacterial culture vibrio fischeri, and Zebrafish to quantify biocompatibility.
From: Multi-Shell Nano-CarboScavengers for Petroleum Spill Remediation
(a) Histogram of percent difference in cell viability from control after CNP-PCDA and NCS exposure. Cell viability was measured via MTT assay in triplicates with a 10,000 cell density sample. (b) Bright field images of morphological and growth density variations in MCF-7 cell culture. (c) IC50 values calculated from MTT triplicate assay with error bars dictating one standard deviation. Stars reveal high statistical significance (student t-test, p-value << 0.001). (d) Microtox EC50 Values measured in triplicates with error bars dictating one standard deviation. Stars reveal high statistical significance (student t-test, p-value << 0.001). (e) Bright field image of zebrafish control post dechorionation (f) Bright field image of dechorionated zebrafish post in ovo treatment with NCS exposed egg water at 10x the calculated IC50 value. (g) Bright field image of dechorionated zebrafish post in ovo treatment with NCS exposed egg water at 30× the calculated IC50 value. (h) Raman overlay analysis of NCS exposed to HMPO over a 168-hour period. (i) Raman overlay analysis of NCS exposed to HRP over an 8-week period. Raman analysis operated at 2% laser power for 1 min with a 532 nm laser.
