Figure 6: Results obtained with birch washing water #C before and after separation by a 100 kDa cut-off centrifugal filter.
From: Boreal pollen contain ice-nucleating as well as ice-binding ‘antifreeze’ polysaccharides
(a) DSC thermograms show the loss of ice nucleation activity in the filtrate sample (bottom panel), while the supernatant (middle panel) shows the same ice nucleation activity as the stock solution (top panel). (b) Ice-shaping experiments show contrary behaviour: the filtrate (bottom panel) shows enhanced ice shaping when compared to the stock solution (top panel); in the supernatant (middle panel) ice shaping is hardly significant. (c) IR spectra of washing water #C (blue) and #B (red) before and after centrifugation. The supernatant spectra (middle panel) differ in the polysaccharide characteristic region and in other additional features, while the filtrate spectra (bottom panel) are very similar.
