Figure 1: Characterization of isolated AEC was performed by immunostaining. | Scientific Reports

Figure 1: Characterization of isolated AEC was performed by immunostaining.

From: Hepatocyte growth factor secreted by bone marrow stem cell reduce ER stress and improves repair in alveolar epithelial II cells

Figure 1

The cells stained for E cadherin performed by immuno-fluroscence (a), the cells were also stained for surfactant protein A, positive cells stain pink by alkaline phosphatase staining (b). Cells also stained for Surfactant protein C (SP-c) as shown with positive immunoflurosence (c). Immunostaining for the ER stress marker GRP78 was performed on the paraffin embedded IPF lung tissue. A distinct staining of the epithelial lining for Grp 78 was seen in area around thickened interstitium. Single mononuclear cell lining the airspaces showed strong staining, based on their location and shape they are likely the alveolar epithelial cells (d). Grp78 positive cells (red) are also positive for SPC (green) thus confirming that AEC are under ER stress in IPF patients (e). (Image processed by Imaris for volume rendering for clear view) (Scale bar (a–c) (50 μm), (d) (500 μm), (e) (10 μm).

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