Figure 1: Platelet-activating factor increases the expression of p21 and acetylated histone-H3.
From: Platelet activating factor-induced expression of p21 is correlated with histone acetylation
Protein expression in HMC-1 cells was analyzed by immunoblotting. (A) p21 expression after 24 h incubation with different concentrations of cPAF, β-actin is the loading control. (B) Acetyl-H3 expression in cells treated with different concentrations of cPAF for 24 h, β-actin is the loading control. (C) p21 expression in normal mast cells harvested 24 h after cPAF treatment, and its corresponding quantification using NIH ImageJ software, p84 is the loading control. (D) Total H3 and acetyl-H3 expression in mast cells treated with 5 and 10 μM cPAF, and its corresponding quantification, p84 is the loading control, *Indicates p < 0.05; One-way ANOVA N = 3. (E) PCNA expression after 24 h incubation with 10 μM cPAF.
