Figure 5: The statiscal hydrogen bond profile among talin2^WT/integrin, talin1^WT/integrin and talin1^C336S/integrin complexes.

(A) The difference of average inter-molecular hydrogen bond number between talin2^WT/integrin and talin1^WT/integrin. As the difference is dependent on the threshold of hydrogen bond definiation, the profile of various hydrogen bond distances and angle cutoffs is shown. Here the hydrogen bond distance is defined as the distance between hydrogen bond donor and acceptor, while the hydrogen bond angle is defined as the angle between donor, hydrogen, and acceptor. For a typical hydrogen bond distance cutoff of 3.0 Å and angle cutoff of 120°, talin2^WT/integrin complex has about 1 more inter-molecular hydrogen bond than talin1^WT/integrin complex. (B) The difference of the average inter-molecular hydrogen bond number between talin2^WT/integrin and talin1^C336S/integrin. It could be observed that these two complexes form an inter-molecular hydrogen bond in a similar level.