Figure 7: Substitution of talin2 S339 with Cys diminished focal adhesion formation.
From: The molecular basis of talin2’s high affinity toward β1-integrin
Talin2-null U2 OS cells were transfected with EGFP-talin2WT and -talin2S339C, respectively, plated on fibronectin (5 μg/ml), fixed with paraformaldehyde, and stained with anti-phospho-FAK[pY397] antibody. Focal adhesions were examined with a TIRF microscopy. Scale bar, 20 μm. (A) The distribution of talin2, talin2S339C, and phospho-FAK in U2 OS cells. (B) Area distribution of phospho-FAK staining in U2 OS cells. Data are mean ± SEM of 3 experiments. In each group, FAs from 20 cells were analyzed and plotted.
