Figure 1: Expression profile and hormone release in the presence or absence of BIM-23A760 in normal pituitaries from baboons and humans. | Scientific Reports

Figure 1: Expression profile and hormone release in the presence or absence of BIM-23A760 in normal pituitaries from baboons and humans.

From: BIM-23A760 influences key functional endpoints in pituitary adenomas and normal pituitaries: molecular mechanisms underlying the differential response in adenomas

Figure 1

(A) Expression profile of sst2, sst5, D2 (Total and Long) receptors in whole pituitary of female baboons (n = 3) and human pituitary (n = 5). (B) Effect of BIM-23A760 (100 nM) after 4-h treatment on the secretion and mRNA expression of all pituitary hormones in baboon primary pituitary cell cultures (n = 3; top and bottom panel on the left, respectively), and 24-h treatment on GH, PRL and ACTH release in human primary pituitary cell culture (n = 1; right-panel). (C) Effect of BIM-23A760 on POU1F1, sst2, sst5, D2 (Total and Long) after 4-h treatment in baboon primary pituitary cell cultures (n = 3). Values in figure B/C are expressed as percent of vehicle-treated controls, set at 100% within experiment. Hormonal release was determined by commercial ELISA kits. mRNA expression levels were measured by qPCR, and mRNA copy numbers were adjusted by Cyclophilin A (PPIA) and beta-actin (ACTB) mRNA copy number expression in baboons and humans, respectively. Values represent the mean ± SEM (3–4 wells/treatment/experiment). Asterisks show significant differences between BIM-23A760 and vehicle-treated controls in figure B and C (*p < 0.05, **p < 0.01; ***p < 0.001). In cases where only one experiment (n = 1) could be performed, no error bars are presented and no significance tests were performed.

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