Table 2 Effect of BIM-23A760 on free cytosolic calcium kinetics.

From: BIM-23A760 influences key functional endpoints in pituitary adenomas and normal pituitaries: molecular mechanisms underlying the differential response in adenomas

 

PRC (%)

PMR (%) ± SEM

Time ± SEM

Normal pituitary-1

60.0

71.2 ± 2.4

109.4 ± 0.5

Normal pituitary-2

52.0

79.1 ± 2.2

74.2 ± 0.7

Normal pituitary-3

16.3

76.0 ± 3.0

59.1 ± 3.4

Normal pituitary-4

37.5

83.2 ± 1.1

69.7 ± 0.3

GHoma-1

52.5

65.0 ± 2.2

38.3 ± 2.4

GHoma-2

83.5

71.9 ± 6.0

42.11 ± 7.2

GHoma-3

97.5

81.3 ± 1.05

30.13 ± 0.97

GHoma-4

90.0

74.2 ± 2.8

30.0 ± 0.6

GHoma-5

80.0

68.8 ± 2.0

42.0 ± 1.7

GHoma-6

76.6

68.3 ± 8.7

47.8 ± 2.4

GHoma-7

77.3

72.5 ± 2.3

25.6 ± 2.0

GHoma-8

87.5

61.1 ± 2.2

54.3 ± 2.0

GHoma-9

65.0

74.8 ± 0.8

104.8 ± 0.2

GHoma-10

58.2

80.6 ± 3.2

38.6 ± 0.3

GHoma-11

20.0

64.8 ± 3.2

36 ± 3.5

GHoma-12

12.5

72.6 ± 4.3

61.0 ± 5.7

GHoma-13

27.5

77.4 ± 1.3

71.4 ± 4.5

GHoma-14*

90.0

243.8 ± 9.1

27.8 ± 1.6

GHoma-15*

89.5

228.4 ± 3.3

18.0 ± 7.1

GHoma-16*

86.3

254.7 ± 15.2

37.0 ± 4.4

GHoma-17*

11.3

123.3 ± 6.7

31.5 ± 3.5

GHoma-18*

97.5

199.5 ± 6.8

26.2 ± 0.7

GHoma-19*

37.5

222.7 ± 13.6

56.3 ± 3.7

GHoma-20†

0.0

  

Mixed GH/PRLoma-1

30.0

76.9 ± 2.0

43.8 ± 2.0

Mixed GH/PRLoma-2

63.9

63.1 ± 2.4

53.0 ± 2.0

Mixed GH/PRLoma-3

32.5

77.0 ± 1.7

77.7 ± 4.5

Mixed GH/PRLoma-4

41.2

81.5 ± 2.4

26.0 ± 3.0

Mixed GH/PRLoma-5†

0.0

  

PRLoma-1

41.0

63.0 ± 3.0

49.0 ± 2.4

PRLoma-2

25.0

59.9 ± 2.8

92.0 ± 4.5

PRLoma-3

42.9

63.0 ± 5.5

48.3 ± 4.9

PRLoma-4

9.5

88.0 ± 1.3

35 ± 0

PRLoma-5†

0.0

  

PRLoma-6†

0.0

  

ACTHoma-1

100.0

58.5 ± 3.8

82.5 ± 8.8

ACTHoma-2

32.5

58.5 ± 4.3

53.1 ± 3.2

ACTHoma-3

15.0

86.5 ± 3.7

48.3 ± 4.9

ACTHoma-4*

2.5

232.7 ± 0.0

20.0 ± 0.0

ACTHoma-5*

15.0

172.0 ± 17.0

28.0 ± 4.5

ACTHoma-6*

55.0

247.7 ± 13.0

67.5 ± 2.4

ACTHoma-7†

0.0

  

ACTHoma-8†

0.0

  

ACTHoma-9†

0.0

  

ACTHoma-10†

0.0

  

NFPA-1

70.7

82.4 ± 0.3

52.9 ± 24.6

NFPA-2

20.5

78.1 ± 2.3

58.4 ± 4.6

NFPA-3

42.5

81.3 ± 8.8

47.8 ± 4.8

NFPA-4

43.5

83.3 ± 0.6

65.0 ± 2.0

NFPA-5*

17.6

163.0 ± 17.0

51.0 ± 2.7

NFPA-6*

20.0

177.1 ± 18.8

30.0 ± 5.0

NFPA-7†

0.0

  

NFPA-8†

0.0

  

NFPA-9†

0.0

  

NFPA-10†

0.0

  

NFPA-11†

0.0

  

NFPA-12†

0.0

  

NFPA-13†

0.0

  

NFPA-14†

0.0

  

NFPA-15†

0.0

  

NFPA-16†

0.0

  

FSHoma-1

66.2

64.5 ± 0.6

52.9 ± 5.7

TSHoma-1

64.7

52.9 ± 3.0

14.0 ± 0.8

TSHoma-2†

0.0

  
  1. The proportion of responsive cells (PRC) showing changes in [Ca2+]i levels in response to BIM-23A760 is indicated for all the pituitary cell culture preparations. Percentage of maximum response (PMR) and time of response to BIM-23A760 administration are also indicated. Pituitary adenomas in which an stimulatory response to BIM-23A760 was observed are indicated by an asterisk (*) while adenomas that did not respond to BIM-23A760 are indicated by a cross (†). The percentage of response (in %) and the time (in seconds) is indicated as mean ± SEM of 20–120 individual cells (depending on the experiment).
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