Figure 3: Immunofluorescence to assess the binding affinity of antibody containing sera with S. globosa. | Scientific Reports

Figure 3: Immunofluorescence to assess the binding affinity of antibody containing sera with S. globosa.

From: Recombinant Phage Elicits Protective Immune Response against Systemic S. globosa Infection in Mouse Model

Figure 3

(a) S. globosa stained with DAPI (excitation: 358 nm, emission: 461 nm), (b) S. globosa incubated with serum containing anti-WP and stained with goat anti-mouse IgG conjugated with Cy3 (excitation: 550 nm, emission: 570 nm), (c) Merged image of (a) and (b). (d) S. globosa stained with DAPI (excitation: 358 nm, emission: 461 nm), (e) S. globosa incubated with sera containing anti-RP (recombinant phage displaying peptide-kpvqhalltplgldr) and stained by goat anti-mouse IgG conjugated with Cy3 (excitation: 550 nm, emission: 570 nm), (f) merged image of (d) and (e). Fluorescence images illustrate binding of the serum antibodies collected from RP-immunized mice to S. globosa. RP: recombinant phage, WP: wild-type phage.

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