Figure 1: ABCG1 organized into filamentous structures at the plasma membrane.
From: The ATP binding cassette transporter, ABCG1, localizes to cortical actin filaments
After membrane removal and imaging of the remaining membrane sheet in control (a) or cholesterol treated (c) cells, the filaments are more visible. (b,d) are examples of 2D ICS auto-correlation functions (ACF) for control and cholesterol treated cells, respectively. Insets in (b,d) display the top view of 2D ACFs, indicating the extent of the 2D variances of these distributions. The 2D variances of ACFs, shown in (e) for cholesterol treated (n = 30 cells) cells show much larger variances than controls (n = 25 cells), indicating that cholesterol increases the filamentation pattern and the spread of 2D ACFs. This is in agreement with much lower 2D kurtosis (peakedness) of cholesterol treatment ACFs, shown in (f). The mean of the data is shown as a red line. The 95% confidence intervals are represented by the pink zones and one standard deviation is represented by the blue zones ****P < 0.0001.
