Figure 5: Chromatin immunoprecipitation evidence the spatial relationship between Rb1 and the TERRA promoter in mouse and human cells. | Scientific Reports

Figure 5: Chromatin immunoprecipitation evidence the spatial relationship between Rb1 and the TERRA promoter in mouse and human cells.

From: The Rb1 tumour suppressor gene modifies telomeric chromatin architecture by regulating TERRA expression

Figure 5

(A) ChIP with anti-Rb1 in Rb1+/+ and Rb1+/Δ19 murine osteoblasts showed that Rb1 haploinsufficiency led to a significant reduction of the endogenous TERRA promoter sequence recovered from murine cells. Error bars show the SD of 3 biological replicates. Student’s t-test was used to assess the significance. (B) Transient transfections in U2OS show that RB1 levels influence the amount of endogenous TERRA promoter recovered after immunoprecipitation with anti-RB1 and anti-pRb1 (Ser 807/811). Error bars show the mean of 3 technical replicates for each antibody. Significance was assessed with the ANOVA test and Student t-test for comparison among the two different transfected cells.

Back to article page