Table 4 Sequences of Primers and siRNAs.
From: GPR155 Serves as a Predictive Biomarker for Hematogenous Metastasis in Patients with Gastric Cancer
Gene | Experiment | Type | Sequence | Product size |
|---|---|---|---|---|
GPR155 | qRT-PCR | forward | 5′-AGCAAAGCTGGACTATTCCCT-3′ | 125 bp |
reverse | 5′-GCCACCAAATAAATGTACTGGA-3′ | |||
Bisulfite | forward | 5′-TTTTTGTTTTTGTTTTTTAGGTTTG-3′ | 197 bp | |
sequencing | reverse | 5′-AACTAAAAATAACAATTCTATCTCC-3′ | ||
Knockdown | siRNA | 5′-ACAUCAUAAGAGAUAUUGG-3′ |  | |
5′-GUACAAUAGAACAAACACC-3′ |  | |||
5′-AUCAAAACUAACAUUCUGG-3′ |  | |||
5′-GUAUAUCACAGCUUCACCA-3′ |  | |||
GAPDH | qRT-PCR | forward | 5′-GAAGGTGAAGGTCGGAGTC-3′ | 226 bp |
probe | 5′-CAAGCTTCCCGTTCTCAGCC-3′ | |||
reverse | 5′-GAAGATGGTGATGGGATTTC-3′ |
