Figure 3: Tim-3 inhibits Nrf2 nuclear translocation.
From: Tim-3 inhibits macrophage control of Listeria monocytogenes by inhibiting Nrf2
A549 cells expressing GFP-labeled Nrf2 were transfected with hTim3-pcDNA3.1 or vector control for 48 h, then were incubated with 20 μM menadione (VK) for 30 min and imaged and analyzed on a GE IN Cell Analyzer 2000. The activation of Nrf2 was expressed as the nucleus/cytosol GFP signal ratio. (A) Representative images showing VK-induced nuclear translocation of Nrf2. (B) Summarized data for the PCTACT in the presence of VK. The results, which are representative of those obtained in three independent assays, are the mean ± SD for triplicate wells; ***p < 0.001. (C) RAW264.7 cells were incubated with 20 μg/ml of sTim-3-Trx (sTim-3) or Trx control (Con) for 15 min, then nuclear and cytoplasmic extract were isolated to analyze for Nrf2 protein level by Western blotting.
