Figure 7: Blockade of Tim-3 in vivo increases CD36 and HO-1 expression by macrophages and attenuates L. monocytogenes infection.
From: Tim-3 inhibits macrophage control of Listeria monocytogenes by inhibiting Nrf2
C57BL/6 mice were injected i.p. with L. monocytogenes (5 × 107 CFU/mouse), then with 200 μg of soluble sTim-3-Trx (sTim-3) or Trx control (Con). Three days later, peritoneal macrophages were isolated and examined for the expression of CD36 mRNA (A up panel) by real-time PCR and protein (A lower panel) by flow cytometry and HO-1 (B) mRNA levels by real-time PCR and the bacterial burden was examined in the peritoneal fluid (C) and spleen homogenate (D). (E) H&E staining of liver sections, which showed scattered, small foci of infection often surrounding a core of dead hepatocytes in the livers of sTim-3-Trx (sTim-3) treated mice compare to those much more numerous, large foci of infection in the livers of Trx control (Con) treated control mice. In (A–D), the data shown are the mean ± SD for a group of 6 mice and are representative of results for two independent experiments using 4 or 6 mice in each group. *p < 0.05; **p < 0.01; ***p < 0.001.
