Figure 2: Salmonella lacking EF-P decreases MgtB protein levels despite increasing MgtC protein levels. | Scientific Reports

Figure 2: Salmonella lacking EF-P decreases MgtB protein levels despite increasing MgtC protein levels.

From: Elongation factor P restricts Salmonella’s growth by controlling translation of a Mg2+ transporter gene during infection

Figure 2

Western blot analysis of crude extracts prepared from either wild-type (14028s) or the efp mutant Salmonella (DN337). Blots were probed with anti-MgtB (A), anti-MgtC (B), anti-EF-P (C), or anti-Fur (D) antibodies to detect MgtB, MgtC, EF-P, and Fur proteins respectively. Bacteria were grown for 5 h in N-minimal media containing 10 mM or 0.01 mM Mg2+ as described in Methods.

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