Figure 3: EF-P is required for MgtB production in a manner dependent on Pro555 and Pro556 codons. | Scientific Reports

Figure 3: EF-P is required for MgtB production in a manner dependent on Pro555 and Pro556 codons.

From: Elongation factor P restricts Salmonella’s growth by controlling translation of a Mg2+ transporter gene during infection

Figure 3

(A) Schematic diagram of the MgtB protein and location of proline codons used in this work. (B–D) Western blot analysis of crude extracts prepared from a strain with the wild-type mgtB gene (14028s), the efp mutant (DN337), an mgtB derivative with the Pro 73, 74 codons substituted by Ala codons (EN793), a mutant with both the Pro 73, 74 substitution and the efp insertion (EN794), an mgtB derivative with the Pro 555, 556 codons substituted by Ala codons (EN873), or a mutant with both the Pro 555, 556 substitution and the efp insertion (EN877). Blots were probed with anti-MgtB (B), anti-MgtC (C), anti-EF-P (D) or anti-Fur (E) antibodies to detect MgtB, MgtC, EF-P and Fur proteins respectively. Bacteria were grown for 5 h in N-minimal media containing 0.01 mM Mg2+ as described in Methods.

Back to article page